Technical Manual

Chapter	Content
Chapter 1-A Standard Cell Panels	Establishment and Use of Standard Cell Panels for Control of HLA Genomic Typing by E Mickelson, revised September 2000
Chapter 1-B SBT Reference Panel	Reference Panels for Sequence Based Typing: Selection Criteria for HLA-A and HLA-B by E Rozemuller
Chapter 1-C Quality Control	Quality Control Test Panels for SSOP & SBT by CK Hurley and J Ng
Chapter 2-A SSOP, Methods/Protocols	Typing for HLA Class I Genes with Sequence Specific Oligonucleotide Probes (SSOP), Methods and Protocols by CK Hurley, M Fernandez-Vina, X Gao, D Middleton, J Ng, H Noreen, EC Ren, B Schmeckpeper, A Smith, T Tang and K Tokunaga
Chapter 2-B-1 SSOP, HLA-A	Typing for Class I HLA-A Alleles with Sequence Specific Oligonucleotide Reagents. Primers and Probes. Version 1.9 - October, 2002 by CK Hurley, M Fernandez-Vina, X Gao, D Middleton, H Noreen, EC Ren, A Smith
Chapter 2-B-2 SSOP, HLA-B	Typing for HLA-B Alleles with Sequence Specific Oligonucleotide Reagents Version 1.8 - October, 2002 by CK Hurley, M Fernandez-Vina, X Gao, D Middleton, H Noreen, EC Ren, and A Smith
Chapter 2-B-3 SSOP, HLA-C	Typing for Class I HLA-C Alleles with Sequence Specific Oligonucleotide Reagents. Primers and Probes Version 1.8 - October, 2002 by CK Hurley, M Fernandez-Vina, X Gao, D Middleton, H Noreen, EC Ren, and A Smith
Chapter 2C SSOP Class II	Class II HLA-Typing Primers and Probes Version 1.5 - December, 2001 by CK Hurley, M Fernandez-Vina, D Middleton, H Noreen, B Schmeckpeper, A Smith and T Tang
Chapter 3 Reverse Line Blot	HLA Class I Reverse Lineblot Typing Protocol by LN Geyer, SJ Mack and HA Erlich
Chapter 4 SSP, Class I Variants	Detection of HLA Class I Expression Variants by PCR-SSP by M Bunce, J Procter, J Cook, C Logan and K Welsh
Chapter 5 Primer Locations	Sequencing-Based Typing, Location of Primers - DPB1 by MGJ Tilanus
Chapter 6 Ambiguities	SBT Resolution and Ambiguities by E Rozemuller
Chapter 7-A SBT HLA-A: Hurley	Sequence-Based Typing for HLA-A: Class I Sequencing Amplification of Exons 2 and 3 Based upon Cereb by CK Hurley
Chapter 7-B SBT HLA-A, Ferrra	Sequence-Based Typing for HLA-A, Long & Medium Size PCR Protocol by C Pera, AM Parodi, and GB Ferrara
Chapter 7-C SBT HLA-A, Blaczyk	Sequence-Based Typing for HLA-A, Separating Alleles by R Blasczyk
Chapter 8-A SBT HLA-B, Hurley	Sequence-Based Typing for HLA-B: Class I Sequencing Amplification of Exons 2 and 3 Based upon Cereb by CK Hurley
Chapter 8-B SBT HLA-B, Ferrara	Sequence-Based Typing for HLA-B: Medium Size PCR Protocol by S Pozzi, GB Ferrara and AM Parodi
Chapter 8-C SBT HLA-B, Voorter	Sequence-Based Typing for HLA-B: Amplification and Sequencing of Exons 2 and 3 Separately by C Voorter and E van den Berg-Loonen
Chapter 9-A SBT, HLA-C, Ferrara	Sequence-Based Typing for HLA-C, Medium Size PCR Protocol by L Delfino, A Morabito, C Pera, and GB Ferrara
Chapter 9-B SBT, HLA-C, Voorter	Sequence-Based Typing for HLA-C, Amplification and Sequencing of Exons 2 & 3 Separately by C Voorter and E van den Berg-Loonen
Chapter 9-C SBT, HLA-C, Petersdorf	PCR and Sequencing Protocol for High Resolution Typing of HLA-C Gene by EW Petersdorf and M Malkki
Chapter 10-A SBT, HLA-DRB, Williams	DRB1/3/5 Locus Sequence Based Allele Identification by J Wu and TM Williams
Chapter 10-B SBT, HLA-DRB, Voorter	Sequence-Based Typing for HLA-DRB1 and DRB3/4/5, Strategy for ALF Express by C Voorter and E van den Berg-Loonen
Chapter 10-C SBT, HLA-DRB, Blasczyk	Sequence-Based Typing for HLA-DRB1, Separating Alleles by R Blasczyk
Chapter 10-D SBT, HLA-DRB1, Sayer	Single Tube PCR Amplification Assays for the Sequencing-Based Typing of HLA-DRB Genes by D Sayer, R Whidborne, D DeSantis, and F Christiansen
Chapter 10-E SBT, HLA-DRB3/4/5, Sayer	Sequence-Based Typing for HLA DRB3/4/5, Simultaneous PCR Amplification & Sequencing
Chapter 10-F SBT, HLA-DRB4, Tilanus	Sequencing Based Typing for HLA-DRB4 Exon 3, Strategy for ABI Equipment by J Bakema, E Rozemuller, A-W van der Zwan and MGJ Tilanus
Chapter 11 SBT, HLA-DQA, Williams	DQA1 Locus Allele Identification by DNA Sequencing by TM Williams and J Wu
Chapter 12-A SBT, HLA-DQB, Tilanus	Sequence-Based Typing for HLA-DQB1, Strategy for ABI Sequencing Equipment by A-W van der Zwan, B Griffity, E Rozemuller, T Williams, and MGJ Tilanus
Chapter 12-B SBT, HLA-DQB, Voorter	Sequence-Based Typing for HLA-DQB1, Strategy for ALF Express by CEM Voorter, MC Kik, and EM van den Berg-Loonen
Chapter 12-C SBT, HLA-DQB, Dunn	Sequencing-Based Typing for HLA-DQB1 by PPJ Dunn and S Day
Chapter 13 SBT, HLA-DPA, Rozemuller	Sequence-Based Typing for HLA-DPA1, Strategy for ABI Sequencing Equipment by E Rozemuller, A-W van der Zwan, and MGJ Tilanus
Chapter 14-A SBT, HLA-DPB, van der Zwan	Sequence-Based Typing for HLA-DPB1, Strategy for ABI Equipment by A-W van der Zwan, E Rozemuller and MGJ Tilanus
Chapter 14-B SBT, HLA-DPB, Voorter	Sequence-Based Typing for HLA-DPB1, Strategy for ALF Express by C Voorter and E van den Berg-Loonen
Chapter 14-C Sambig. DPB, van der Zwan	DPB1 Ambiguities Resolved with the Group Specific Amplification Primer Approach by A-W van der Zwan, E Rozemuller and MGJ Tilanus
Chapter 15 mRNA SBT HLA-C, Fischer	cDNA Typing of HLA-C Alleles by Automated Nucleotide Sequencing by I Fae and GF Fischer
Chapter 16 Microsatellites, Carrington	Typing for Microsatellite Markers within the HLA System: Analysis of Recombination and Application to Transplantation by MP Martin and M Carrington, revised August 2002
Chapter 17 Microsatellites, Lie	The Genetics of Disease Susceptibility, Strategies for Analyzing Polygenic HLA Associations Using Microsatellite Markers by BA Lie, DE Undlien, and E Thorsby, revised August 2002
Chapter 18 LOH in Cancer, Garrido	Molecular Strategies to Define HLA Haplotype Loss Associated with Loss of Heterozygosity (LOH) in Human Tumors by I Maleno, T Cabrera, MA Lopez-Nevot, and F Garrido
Chapter 19 SNPs	Identification of Single Nucleotide Polymorphisms (SNPs) Within the HLA System
Chapter 20 RSCA	Typing for HLA Alleles by Reference Strand Mediated Conformation Analysis (RSCA) by JR Arguello, B Soteriou, A McWhinnie, C Garcia, M Perez-Rodriguez, A Corell, SGE Marsh, A-M Little and JA Madrigal
Chapter 21 DNA Arrays	High Resolution HLA Typing for Large Populations: Development of Multiple DNA Arrays for Identification of HLA Alleles
Chapter 22 Peptide Ligands	Analysis of HLA Specificity for Peptide Ligands
Chapter 23 Statistical Methods	Human Diversity and Variation: Mathematical Tools and Models for Defining Relationships between Different Populations by G Thomson, D Meyer, H Erlich, and S Mack
Chapter 24 Serology Dictionary	HLA Serology to DNA Equivalents Dictionary by GM Schreuder
Chapter 25 Informatics	Informatics, Data Storage & Utilization in the XIII International HLA Workshop by W Helmberg, and E Rozemuller
Chapter 26 HLA E, F and G	HLA E, F and G Typing Strategies
Chapter 27 MIC Genes	MIC A and MIC B Allele Determination by Sequencing-Based Typing (SBT) by M Ota, Y Katsuyama, S Bahram, T Naruse, and H Inoko
Chapter 28 Mono-Allelic Ampl, Dormoy	Mono-Allelic Amplifications of Exons 2 to 4 Using Allele Group-Specific Primers for HLA-A*, B* and Cw* Genes, Sequence-Based Typing by A Dormoy, N Froelich, R Leisenbach, B Weschler and M-M Tongio
Chapter 29 B-locus Ambiguity, Burdett	Resolution of B-locus Sequencing-Based Typing Ambiguities Using Group Specific Sequencing Primers by L Burdett, K Cao, and M Fernandez-Vina
Chapter 30-A SBT, HLA-A, B, C, Dunn	Sequencing-Based Typing for HLA-A, B and C by PPJ Dunn and S Day
Chapter 30-B SBT, HLA-A, B, C, Dunn	Sequencing of the 5' Untranslated Region of HLA Class I Alleles by PPJ Dunn and S Day