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Steps to Follow
1) Enroll in the workshop using the enrollment
form.
2) Order your reagents. For high resolution HLA-A, B, C, DR, DQ, DP SSOP
typing, workshop approved standardized reagents can be ordered from the
SSOP provider. A complete listing
of SSOP primers and probes can be found on the SSOP
protocol pages. For high resolution HLA typing reagents using sequencing-based
typing methods, standardized SBT primers can be ordered from the SBT
Provider. For detection of HLA-A, B, C null alleles using the sequence-specific
priming method, standardized SSP primers can be ordered from the SSP
Provider.
If you will be using reverse line strip (RLS) typing (Anthropology only),
RLS reagents can be ordered from Roche Molecular
Systems. If you are interested in other typing methods (e.g., microsatellite
testing, single nucleotide polymorphism typing), please contact the project
leaders directly.
3) Order your reference panel. Reference panels of DNA for use as positive
and negative primer and probe controls for SSOP typing (Panel
1) and SBT typing (Panel
2) will be available from the Cell and Gene Bank Reference/QC
Order Form (PDF document for download). An announcement will be sent
to all participants when these panels are available.
4) In order to participate in workshop studies, you will need to select
specific workshop projects. Consult the Table of Technology
for information about the typing methods and level of resolution used
by each project. Please contact project leaders directly with specific
questions about typing methods and protocols.
5) Establish a typing system in your laboratory and then validate the
system with reference cells.
6) In order to submit data to the workshop you will need to pass a qualification
test (more information here).
Laboratories achieving greater than 92% accuracy for each locus typed
will be approved for data entry. You can request a qualification test
panel of 25 unknown cells using the same order form used for the Cell
and Gene Bank Reference/QC Order Form (PDF format
download). Please do not request these QC cells until you have established
the typing system in your laboratory.
7) Type the qualification panel using the appropriate typing method and
submit data to the Quality Control Laboratory using the data submission
form (being revised and fine-tuned / available in the very near future).
The data submission form is in Excel format; if you do not have this available
on your computer, please contact gschoch@fhcrc.org
(Gary Schoch) for an alternative method of data submission.
8) When your laboratory has successfully qualified for workshop studies,
project leaders will contact you with specific instructions for participation
in workshop projects that you have selected.
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