SBT Protocols

HLA-A

Gene and primer location

HLA-A Ambiguities List

Chapter 7-A: Class I Sequencing Protocol- Amplification of Exons 2 and 3 Based Upon Cereb, by Carolyn Hurley

Chapter 7-B: Long and Medium Size PCR Protocol, by C. Pera, A.M. Parodi and G.B. Ferrara

Chapter 7-C: Separating Alleles, by Rainer Blasczyk

HLA-B

Gene and primer location

HLA-B Ambiguities List

Chapter 8-A: Class I Sequencing Protocol- Amplification of Exons 2 and 3 Based Upon Cereb, by Carolyn Hurley

Chapter 8-B: Medium Size PCR Protocol, by S. Pozzi, G. B. Ferrara, and A.M. Parodi

Chapter 8-C: Amplification and Sequencing of Exons 2 and 3 Separately, by Christine Voorter and Ella van den Berg-Loonen

HLA-C

Gene and primer location

HLA-C Ambiguities List

Chapter 9-A: Medium Size PCR Protocol, by L. Delfino, A. Morabito, C. Pera and G. B. Ferrara

Chapter 9-B: Amplification and Sequencing of Exons 2 and 3 Separately, by Christine Voorter and Ella van den Berg-Loonen

Chapter 15: cDNA Typing of HLA-C Alleles by Automated Nucleotide Sequencing, by I. Fae and G. F. Fischer

Chapter 9-C: PCR and Sequencing Protocol for High Resolution Typing of HLA-C Gene, by E. Petersdorf, and M. Malkki

HLA-DR

Gene and primer location

HLA-DRB1 Ambiguities List

HLA-DRB 3, 4, 5 Ambiguities List

Chapter 10-A: DRB1/3/5 Locus Sequence Based Allele Identification, by Jin Wu and Thomas Williams

Chapter 10-B: Sequence-Based Typing for HLA-DRB1 and DRB 3/4/5, Strategy for ALF Express, by Christine Voorter and Ella van den Berg-Loonen

Chapter 10-C: Sequencing-Based Typing for HLA-DRB1, Separating Alleles, by Rainer Blasczyk

Chapter 10-D: Single Tube PCR Amplification Assays for the Sequencing-Based Typing of HLA-DRB Genes, by D. Sayer, R. Whidborne, D. DeSantis, and F. Christiansen

Chapter 10-F: Sequencing Based Typing for HLA-DRB4 exon 3. Strategy for ABI Equipment, by J. Bakema, E. Rozemuller, A. van der Zwan, and M.G.J. Tilanus

HLA-DQA

Gene and primer location

Chapter 11: DQA1 Locus Allele Identification by DNA Sequencing, by T. Williams and J, Wu

HLA-DQB

Gene and primer location

HLA-DQB1 Ambiguities List

Chapte 12-A: Sequence-Based typing for HLA-DQB1. Strategy for ABI Sequencing Equipment, by A. van der Zwan, B. Griffith, E. Rozemuller, T. Williams, and M.G.J. Tilanus

Chapter 12-B: Sequence-Based Typing for HLA-DQB1. Strategy for ALF Express by C. Voorter, M. Kik, E.M. van den Berg-Loonen

Chapter 12-C: Sequencing-Based Typing for HLA-DQB1 by P. Dunn and S. Day

HLA-DPA

Gene and primer location

HLA-DPA1 Ambiguities List

Chapter 13: Sequence-Based Typing for HLA-DPA1. Strategy for ABI Sequencing Equipment by E. Rozemuller, A van der Zwan and M.G.J. Tilanus

HLA-DPB

Gene and primer location (excel file)

HLA-DPB1 Ambiguities List

Chapter 14-A: Sequence-Based Typing for HLA-DPB1. Strategy for ABI Equipment by A. van der Zwan, E. Rozemuller and M.G.J. Tilanus

Chapter 14-B: Sequence-Based Typing for HLA-DPB1. Strategy for ALF Express by C. Voorter and E. van den Berg-Loonen

Chapter 14-C: DPB1 Ambiguities Resolved with the Group Specific Amplification Primer Approach by A. van der Zwan, E. Rozemuller and M.G.J. Tilanus

MIC A and MIC B

Gene and primer location (excel file)

Chapter 27: MIC A and MIC B Allele Determination by Sequencing-Based Typing. by M. Ota, Y. Katsuyama, S. Bahram, T. Naruse, and H. Inoko

Others

Gene and primer location (excel file)

Ambiguities (pdf download)

Allele Database