After reviewing the HLA-C locus QC data submitted from several labs, we have concluded that probe 14 on the HLA-C strip has been the source of much of the difficulty experienced carrying out HLA-C QC typings. It is imperative that you carefully consider this probe when entering your data using the pattern interpretation software. ANY signal at probe 14, no matter how faint, should be entered as positive during future Workshop typing.
For comparison, please examine the figure below, which shows a set of seven HLA-C locus strips. Though faint, probe 14 is positive on strips 1, 3, 4, and 5.
